Modulation of heat shock protein expression and cytokine levels in MCF‐7 cells through photodynamic therapy

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dc.contributor.authorSantos, Mariela Inês Batista dos
dc.contributor.authorGodoi, Bruno Henrique
dc.contributor.authorSilva, Newton Soares da
dc.contributor.authorOliveira, Luciane Dias de
dc.contributor.authorRamos, Lucas de Paula
dc.contributor.authorCintra, Ricardo Cesar
dc.contributor.authorPacheco‐Soares, Cristina
dc.date.accessioned2025-02-11T20:26:35Z
dc.date.available2025-02-11T20:26:35Z
dc.date.issued22024-05-24
dc.description.abstractIn this study, we assess the impact of photodynamic therapy (PDT) using aluminum phthalocyanine tetrasulfonate (AlPcS4) on the viability and cellular stress responses of MCF-7 breast cancer cells. Specifically, we investigate changes in cell viability, cytokine production, and the expression of stress-related genes. Experimental groups included control cells, those treated with AlPcS4 only, light-emitting diode (LED) only, and combined PDT. To evaluate these effects on cell viability, cytokine production, and the expression of stress-related genes, techniques such as 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide (MTT) assay, enzyme-linked immunosorbent assays (ELISA), and real-time quantitative PCR (RT‒qPCR) were employed. Our findings reveal how PDT with AlPcS4 modulates mitochondrial activity and cytokine responses, shedding light on the cellular pathways essential for cell survival and stress adaptation. This work enhances our understanding of PDT's therapeutic potential and mechanisms in treating breast cancer.
dc.description.physical8 f.
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipFinanciadora de Estudos e Projetos (Finep)
dc.description.uriFAPESP grant number 2016/17984–1; 2021/09418–4 FINEP grant number 01.18.0053.00
dc.format.mimetypepdf
dc.identifier.affiliationUniversidade do Vale do Paraíba
dc.identifier.affiliationUniversidade do Vale do Paraíba
dc.identifier.affiliationUniversidade Estadual Paulista Júlio de Mesquita Filho
dc.identifier.affiliationUniversidade Estadual Paulista Júlio de Mesquita Filho
dc.identifier.affiliationUniversidade Estadual Paulista Júlio de Mesquita Filho
dc.identifier.affiliationUniversidade de São Paulo
dc.identifier.affiliationUniversity Claude Bernard Lyon
dc.identifier.affiliationUniversidade do Vale do Paraíba
dc.identifier.bibliographicCitationDOS SANTOS, M. I. B. et al. Modulation of heat shock protein expression and cytokine levels in MCF-7 cells through phomic therapy. Lasers in Medical Science, [S. l.], v. 39, n. 1, p. 135, 24 maio 2024. Disponível em: https://link.springer.com/10.1007/s10103-024-04092-1.
dc.identifier.doi10.1007/s10103-024-04092-1
dc.identifier.urihttps://repositorio.univap.br/handle/123456789/567
dc.language.isoen_US
dc.publisherSpringer-Verlag London Ltd.
dc.rights.holderSpringer-Verlag London Ltd.
dc.subject.keywordPhotodynamic therapy
dc.subject.keywordMTT assay
dc.subject.keywordCell viability
dc.subject.keywordCytokines
dc.subject.keywordGene expression
dc.subject.keywordHeat shock proteins
dc.titleModulation of heat shock protein expression and cytokine levels in MCF‐7 cells through photodynamic therapy
dc.typeArtigos de Periódicos

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