Oxidative cleavage of polysaccharides by a termite-derived superoxide dismutase boosts the degradation of biomass by glycoside hydrolases

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dc.contributor.authorCairo, João Paulo L. Franco
dc.contributor.authorMandelli, Fernanda
dc.contributor.authorTramontina, Robson
dc.contributor.authorCannella, David
dc.contributor.authorParadisi, Alessandro
dc.contributor.authorCiano, Luisa
dc.contributor.authorFerreira, Marcel R.
dc.contributor.authorLiberato, Marcelo V.
dc.contributor.authorBrenelli, Lívia B.
dc.contributor.authorGonçalves, Thiago A.
dc.contributor.authorRodrigues, Gisele N.
dc.contributor.authorAlvarez, Thabata M.
dc.contributor.authorMofatto, Luciana S.
dc.contributor.authorCarazzolle, Marcelo F.
dc.contributor.authorPradella, Jose Geraldo da Cruz
dc.contributor.authorLeme, Adriana F. Paes
dc.contributor.authorLeonardo, Ana M. Costa
dc.contributor.authorNeto, Mário Oliveira
dc.contributor.authorDamasio, André
dc.contributor.authorDavies, Gideon J.
dc.contributor.authorFelby, Claus
dc.contributor.authorWalton, Paul H.
dc.contributor.authorSquina, Fabio M.
dc.date.accessioned2025-04-02T19:10:16Z
dc.date.available2025-04-02T19:10:16Z
dc.date.issued22022-05-12
dc.description.abstractWood-feeding termites effectively degrade plant biomass through enzymatic degradation. Despite their high efficiencies, however, individual glycoside hydrolases isolated from termites and their symbionts exhibit anomalously low effectiveness in lignocellulose degradation, suggesting hereto unknown enzymatic activities in their digestome. Herein, we demonstrate that an ancient redox-active enzyme encoded by the lower termite Coptotermes gestroi, a Cu/Zn superoxide dismutase (CgSOD-1), plays a previously unknown role in plant biomass degradation. We show that CgSOD-1 transcripts and peptides are up-regulated in response to an increased level of lignocellulose recalcitrance and that CgSOD-1 localizes in the lumen of the fore- and midguts of C. gestroi together with termite main cellulase, CgEG-1-GH9. CgSOD-1 boosts the saccharification of polysaccharides by CgEG-1-GH9. We show that the boosting effect of CgSOD-1 involves an oxidative mechanism of action in which CgSOD-1 generates reactive oxygen species that subsequently cleave the polysaccharide. SOD-type enzymes constitute a new addition to the growing family of oxidases, ones which are up-regulated when exposed to recalcitrant polysaccharides, and that are used by Nature for biomass degradation.
dc.description.physical14 p.
dc.format.mimetypepdf
dc.identifier.affiliationDepartment of Biochemistry and Tissue Biology, Institute of Biology, University of Campinas (UNICAMP)
dc.identifier.affiliationBrazilian Biorenewables National Laboratory, Brazilian Center for Research in Energy and Materials, Campinas
dc.identifier.affiliationPrograma de Processos Tecnológicos e Ambientais da Universidade de Sorocaba (UNISO)
dc.identifier.affiliationDepartment of Geosciences and Natural Resource Management, Faculty of Science, University of Copenhagen
dc.identifier.affiliationDepartment of Chemistry, University of York
dc.identifier.affiliationDepartment of Chemistry, University of York
dc.identifier.affiliationDepartamento de Física e Biofísica, Instituto de Biociências, Universidade Estadual Paulista, UNESP
dc.identifier.affiliationUniversidade do Vale do Paraíba
dc.identifier.bibliographicCitationFRANCO CAIRO, J. P. L. et al. Oxidative cleavage of polysaccharides by a termite-derived superoxide dismutase boosts the degradation of biomass by glycoside hydrolases. Green Chemistry, [S. l.], v. 24, n. 12, p. 4845–4858, 2022. Disponível em: https://xlink.rsc.org/?DOI=D1GC04519A.
dc.identifier.doiDOI https://doi.org/10.1039/D1GC04519A
dc.identifier.urihttps://repositorio.univap.br/handle/123456789/799
dc.language.isoen
dc.publisherRoyal Society of Chemistry
dc.rights.holderJoão Paulo L. Franco Cairo
dc.rights.holderFernanda Mandelli
dc.rights.holderRobson Tramontina
dc.rights.holderDavid Cannella
dc.rights.holderAlessandro Paradisi
dc.rights.holderLuisa Ciano
dc.rights.holderMarcel R. Ferreira
dc.rights.holderMarcelo V. Liberato
dc.rights.holderLívia B. Brenelli
dc.rights.holderThiago A. Gonçalves
dc.rights.holderGisele N. Rodrigues
dc.rights.holderThabata M. Alvarez
dc.rights.holderLuciana S. Mofatto
dc.rights.holderMarcelo F. Carazzolle
dc.rights.holderJosé G. C. Pradella
dc.rights.holderAdriana F. Paes Leme
dc.rights.holderAna M. Costa-Leonardo
dc.rights.holderMário Oliveira-Neto
dc.rights.holderAndré Damasio
dc.rights.holderGideon J. Davies
dc.rights.holderClaus Felby
dc.rights.holderPaul H. Walton
dc.rights.holderFabio M. Squina
dc.subject.keywordWood-feeding termites
dc.subject.keywordLignocellulose degradation
dc.subject.keywordGlycoside hydrolases
dc.subject.keywordPlant biomass conversion
dc.titleOxidative cleavage of polysaccharides by a termite-derived superoxide dismutase boosts the degradation of biomass by glycoside hydrolases
dc.typeArtigos de Periódicos

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