Amniotic membrane modulates MMP9 and MMP12 gene and protein expression in experimental model of the hepatic fibrosis

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dc.contributor.authorAlves, Ana Paula da Silva
dc.contributor.authorTeixeira, Roberta Jenniffer Maciel
dc.contributor.authorSilva, Raissa Monteiro da
dc.contributor.authorCanevari, Renata de Azevedo
dc.contributor.authorSant’Anna, Luciana Barros
dc.date.accessioned2026-01-21T16:50:34Z
dc.date.available2026-01-21T16:50:34Z
dc.date.issued22024
dc.description.abstractHepatic fibrosis is characterized by excessive deposition of collagen in the hepatic parenchyma, which disturbs the normal architecture and function. We have shown that human amniotic membrane (AM) can be used as a patch on the whole liver surface, resulting in an extremely significant reduction in collagen deposition. The aim of this study was to investigate the effects of AM on the matrix metalloproteinase 9 (MMP9) and matrix metalloproteinase 12 (MMP12) genes and proteins expression by real time quantitative PCR and immunohistochemistry, respectively, as well as image analysis on biliary fibrosis induced in rats by the bile duct ligation (BDL).Two weeks after the BDL, an AM fragment was applied onto the liver, and four weeks later, the liver samples were collected. MMP9 and MMP12 genes were significantly over expressed in group treated with AM. The immunoexpression of MMP9 and MMP12 was observed in all groups. However, the quantitative image analysis demonstrated an increase of the area occupied only by MMP12 in the livers of AM-treated rats with respect to BDL rats. These findings suggest that the AM exerts its beneficial effects on biliary fibrosis by increasing the MMP12, which in turn reduces the excessive collagen deposition on liver tissue.
dc.description.physical12 p.
dc.format.mimetypePDF
dc.identifier.affiliationUniversidade do Vale do Paraíba
dc.identifier.bibliographicCitationALVES, A. P. S. et al. Amniotic membrane modulates MMP9 and MMP12 gene and protein expression in experimental model of the hepatic fibrosis. Anais da Academia Brasileira de Ciências, v. 96, n. 4, p. 1-12, 2024. Disponível em: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652024000600605&tlng=en.
dc.identifier.doi10.1590/0001-3765202420231086
dc.identifier.urihttps://repositorio.univap.br/handle/123456789/1102
dc.language.isoen_US
dc.publisherAcademia Brasileira de Ciências
dc.rights.holderAnais da Academia Brasileira de Ciências
dc.subject.keywordAmniotic membrane
dc.subject.keywordFibrosis
dc.subject.keywordImmunohistochemistry
dc.subject.keywordGene expression
dc.titleAmniotic membrane modulates MMP9 and MMP12 gene and protein expression in experimental model of the hepatic fibrosis
dc.typeArtigos de Periódicos

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