Cellular and metabolic changes after photodynamic therapy in leishmania promastigotes

Página do item simplificado
dc.contributor.authorMarcolino, Luciana Maria Cortez
dc.contributor.authorPereira, André Henrique Correia
dc.contributor.authorPinto, Juliana Guerra
dc.contributor.authorMamone, Leandro Ariel
dc.contributor.authorFerreira-Strixino, Juliana
dc.date.accessioned2025-06-12T15:07:54Z
dc.date.available2025-06-12T15:07:54Z
dc.date.issued22021
dc.description.abstractLeishmaniasis is a zoonotic disease, regarded by WHO as a public health problem that has presented a significant increase in the recent years. Conventional treatment is toxic and leads to serious side effects. Photodynamic therapy has been studied as a treatment to cutaneous leishmaniasis. This study aimed to evaluate the cell viability, morphological changes, type of cell death, production of reactive oxygen species, and changes in the mitochondrial membrane and DNA fragmentation in Leishmania braziliensis and Leishmania major promastigotes. Confocal microscopy was used to quantify the fluorescence emitted by JC-1, Annexin V, and propidium iodide reagents. The trypan blue exclusion test was used to evaluate the viability of the cells, the mitochondrial activity was verified with MTT, and the morphological changes were analyzed for SEM and DNA damage using the comet assay. PDT using curcumin at 500, 125, and 31,25 μg/mL decreased the viability of the parasites and induced changes in the mitochondrial membrane potential. The production of reactive oxygen species was dose- dependent and was observed only in the groups submitted to PDT. DNA damage was also observed in the parasite cells. The morphology of the cells was affected mainly at the highest curcumin concentration, resulting in rounded cells with a shortened flagellum. When the type of cell death was analyzed, the prevalence of apoptosis was noted. The results support the use of curcumin as photosensitizer in PDT against Leishmania promastigotes in the treatment for cutaneous leishmaniasis.
dc.description.physical9 p.
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipFinanciadora de Estudos e Projetos (Finep)
dc.description.uriFAPESP (2016/12244-1; -2013/07276-1) FINEP (01.13.0275.00) CAPES ( Financing Code 001)
dc.format.mimetypePDF
dc.identifier.affiliationUniversidade do Vale do Paraíba
dc.identifier.affiliationUniversidad de Buenos Aires
dc.identifier.bibliographicCitationMARCOLINO, L. M. C. et al. Cellular and metabolic changes after photodynamic therapy in leishmania promastigotes. Photodiagnosis and Photodynamic Therapy, v. 35, p. 1-8, 2021. Disponível em: https://linkinghub.elsevier.com/retrieve/pii/S1572100021002301.
dc.identifier.doi10.1016/j.pdpdt.2021.102403
dc.identifier.urihttps://repositorio.univap.br/handle/123456789/994
dc.language.isoen_US
dc.publisherElsevier
dc.rights.holderPhotodiagnosis and Photodynamic Therapy
dc.subject.keywordCurcumin
dc.subject.keywordPDT
dc.subject.keywordLeishmania
dc.subject.keywordReactive species
dc.subject.keywordMitochondrial membrane potential
dc.titleCellular and metabolic changes after photodynamic therapy in leishmania promastigotes
dc.typeArtigos de Periódicos

Arquivos

Pacote Original
Agora exibindo 1 - 1 de 1
Nenhuma Miniatura disponível
Nome:
Cellular and metabolic changes after photodynamic therapy in leishmania promastigotes.pdf
Tamanho:
3.55 MB
Formato:
Adobe Portable Document Format
Descrição:
Acesso via Portal Capes
Baixar
Licença do Pacote
Agora exibindo 1 - 1 de 1
Nenhuma Miniatura disponível
Nome:
license.txt
Tamanho:
1.71 KB
Formato:
Item-specific license agreed upon to submission
Descrição:
Baixar

Coleções

Produção acadêmica-PPGEB