Influence of Hydrocortisone in Chemotherapy and Photodynamic Therapy in HEp-2 Cells

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dc.contributor.authorMoraes, Carlos Dailton Guedes de Oliveira
dc.contributor.authorGodoi, Bruno Henrique
dc.contributor.authorSilva, Newton Soares da
dc.contributor.authorSoares, Cristina Pacheco
dc.date.accessioned2025-03-25T19:39:05Z
dc.date.available2025-03-25T19:39:05Z
dc.date.issued22022
dc.description.abstractAim: Cancer cells exhibit resistance to the immune response by regulating and altering the expression of mediators responsible for immune cell recruitment and disease progression. Cortisol is a natural hormone that may be associated with diseases such as cancer by stimulating stress and altering the cellular environment, favoring uncontrolled division and contributing to the inhibition of the immune response. In contrast, current therapeutic strategies do not present significant concerns about stress as a variable in cancer diagnosis and prognosis. The response of HEp-2 cells to stress induced by hydrocortisone and to treatment with Cyclophosphamide (CP) and Photodynamic Therapy (PDT) was analyzed. Methods: One mM of hydrocortisone induced stress in the cells. Cells were treated with 200 μg/ mL of cyclophosphamide or Aluminum Phthalocyanine Tetrasulfonate (AlPcS4) photosensitizer, LED irradiation (660 nm wavelength), intensity of 25 mW/cm2, power of 70 mW, fluence of 5 J/ cm2, characterizing the PDT. All groups were evaluated after 24 h and 48 h. Results: Assessment of stress-inducing mitochondrial activity and cell viability were performed, and the results demonstrated that hydrocortisone significantly altered the rate of cell death, compromising the effects of CP. Conclusion: However, hydrocortisone did not change the cell death rates caused by PDT, indicating the possibility of this hormone as an alternative therapy.
dc.description.physical7 p.
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.uriFAPESP (2016/17984-1 and 305920/2017-0) CAPES (888887.179784/2018-00, Finance Code 001)
dc.format.mimetypePDF
dc.identifier.affiliationUniversidade do Vale do Paraíba
dc.identifier.affiliationUniversidade Estadual Paulista
dc.identifier.bibliographicCitationMORAES, Carlos Dailton Guedes de Oliveira et al. Influence of Hydrocortisone in Chemotherapy and Photodynamic Therapy in HEp-2 Cells. Clinics in Oncology, v. 7, n. 1956, p. 1-7, 2022. Disponível em:
dc.identifier.issn2474-1663
dc.identifier.urihttps://repositorio.univap.br/handle/123456789/767
dc.language.isoen_US
dc.publisherClinics in Oncology
dc.rights.holderClinics in Oncology
dc.subject.keywordCancer
dc.subject.keywordStress
dc.subject.keywordMTT assay
dc.subject.keywordPhotodynamic Therapy
dc.titleInfluence of Hydrocortisone in Chemotherapy and Photodynamic Therapy in HEp-2 Cells
dc.typeArtigos de Periódicos

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