Antimicrobial Photodynamic Therapy Mediated by Fotenticine and Methylene Blue on Planktonic Growth, Biofilms, and Burn Infections of Acinetobacter baumannii

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dc.contributor.authorFigueiredo-Godoi, Lívia Mara Alves
dc.contributor.authorGarcia, Maíra Terra
dc.contributor.authorPinto, Juliana Guerra
dc.contributor.authorFerreira-Strixino, Juliana
dc.contributor.authorFaustino, Eliseu Gabriel
dc.contributor.authorPedroso, Lara Luise Castro
dc.contributor.authorJunqueira, Juliana Campos
dc.date.accessioned2025-03-26T18:16:33Z
dc.date.available2025-03-26T18:16:33Z
dc.date.issued22022-05-04
dc.description.abstractAntimicrobial photodynamic therapy (aPDT) is considered a promising alternative strategy to control Acinetobacter baumannii infections. In this study, we evaluated the action of aPDT mediated by a new photosensitizer derivative from chlorin e-6 (Fotoenticine—FTC) on A. baumannii, comparing its effects with methylene blue (MB). For this, aPDT was applied on A. baumannii in planktonic growth, biofilms, and burn infections in Galleria mellonella. The absorption of FTC and MB by bacterial cells was also evaluated using microscopic and spectrophotometric analysis. The results of planktonic cultures showed that aPDT reduced the number of viable cells compared to the non-treated group for the reference and multidrug-resistant A. baumannii strains. These reductions varied from 1.4 to 2 log10 CFU for FTC and from 2 log10 CFU to total inhibition for MB. In biofilms, aPDT with MB reduced 3.9 log10 CFU of A. baumannii, whereas FTC had no effect on the cell counts. In G. mellonella, only MB-mediated aPDT had antimicrobial activity on burn injuries, increasing the larvae survival by 35%. Both photosensitizers were internalized by bacterial cells, but MB showed a higher absorption compared to FTC. In conclusion, MB had greater efficacy than FTC as a photosensitizer in aPDT against A. baumannii.
dc.description.physical16 p.
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.uriCNPq: number 408369/2018-3
dc.format.mimetypepdf
dc.identifier.affiliationUniversidade Estadual Paulista
dc.identifier.affiliationUniversidade Estadual Paulista
dc.identifier.affiliationUniversidade do Vale do Paraíba
dc.identifier.affiliationUniversidade do Vale do Paraíba
dc.identifier.affiliationUniversidade Estadual Paulista
dc.identifier.affiliationUniversidade Estadual Paulista
dc.identifier.affiliationUniversidade Estadual Paulista
dc.identifier.bibliographicCitationFIGUEIREDO-GODOI, L. M. A. et al. Antimicrobial Photodynamic Therapy Mediated by Fotenticine and Methylene Blue on Planktonic Growth, Biofilms, and Burn Infections of Acinetobacter baumannii. Antibiotics, [S. l.], v. 11, n. 5, p. 619, 4 maio 2022. Disponível em: https://www.mdpi.com/2079-6382/11/5/619.
dc.identifier.doi10.3390/antibiotics11050619
dc.identifier.urihttps://repositorio.univap.br/handle/123456789/776
dc.language.isoen_US
dc.publisherMDPI
dc.rights.holderLívia Mara Alves Figueiredo-Godoi
dc.rights.holderMaíra Terra Garcia
dc.rights.holderJuliana Guerra Pinto
dc.rights.holderJuliana Ferreira‐Strixino
dc.rights.holderEliseu Gabriel Faustino
dc.rights.holderLara Luise Castro Pedroso
dc.rights.holderJuliana Campos Junqueira
dc.rights.holderMDPI
dc.subject.keywordAcinetobacter baumannii
dc.subject.keywordPhotodynamic therapy
dc.subject.keywordChlorin
dc.subject.keywordFotoenticine
dc.subject.keywordMethylene blue
dc.subject.keywordBurns
dc.subject.keywordGalleria mellonella
dc.titleAntimicrobial Photodynamic Therapy Mediated by Fotenticine and Methylene Blue on Planktonic Growth, Biofilms, and Burn Infections of Acinetobacter baumannii
dc.typeArtigos de Periódicos

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