Photodynamic therapy of cationic and anionic BSA-curcumin nanoparticles on amastigotes of Leishmania braziliensis and Leishmania major and Leishmania amazonensis

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dc.contributor.authorMarcolino, Luciana Maria Cortez
dc.contributor.authorAmbrósio, Jéssica Aparecida Ribeiro
dc.contributor.authorPinto, Juliana Guerra
dc.contributor.authorFerreira, Isabelle
dc.contributor.authorSimioni, Andreza Ribeiro
dc.contributor.authorFerreira-Strixino, Juliana
dc.date.accessioned2024-07-23T16:55:59Z
dc.date.available2024-07-23T16:55:59Z
dc.date.issued22024-04
dc.description.abstractCutaneous leishmaniasis is a neglected disease prevalent in tropical countries, and conventional treatment can cause several serious side effects. Photodynamic therapy (PDT) can be considered a promising treatment alternative, as it is non-invasive therapy that has no side effects and uses accessible and low-cost substances, such as curcumin. This study evaluated the PDT response with cationic and anionic BSA nanoparticles encapsulated with curcumin in macrophages infected with L. braziliensis, L. major, and L. amazonensis. The nanoparticle system was characterized using a steady-state technique, scanning electron microscopy (SEM) study, and its biological activity was evaluated using macrophage cell lines infected with different Leishmania species. All spectroscopy measurements demonstrated that BSA curcumin (BSACur) has good photophysical properties, and confocal microscopy shows that macrophages and protozoa internalized the nanoparticles. The viability test demonstrated that at low concentrations, such as 0.1, 0.7, and 1.0 μmol. L 1, there was a decrease in cell viability after PDT application. Furthermore, a decrease in the number of parasites recovered was observed in the PDT groups. The results allowed us to conclude that curcumin loaded into BSA nanoparticles may have potential application in drug delivery systems for PDT protocols, demonstrating reduced cell viability at lower concentrations than free curcumin.
dc.description.physical10 p.
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipFinanciadora de Estudos e Projetos (Finep)
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.uriFapesp 2016/12211–4 CNPq 406282/2021–8 CNPq) 303178/2021–3 FAPESP/CEPOF-2013/07276–1, FINEP 01.13.0275.00
dc.format.mimetypepdf
dc.identifier.affiliationUniversidade do Vale do Paraíba
dc.identifier.bibliographicCitationMARCOLINO, L. M. C. et al. Photodynamic therapy of cationic and anionic BSA-curcumin nanoparticles on amastigotes of Leishmania braziliensis and Leishmania major and Leishmania amazonensis. Photodiagnosis and Photodynamic Therapy, [s. l.], v. 46, p. 104001, 2024.
dc.identifier.doi10.1016/j.pdpdt.2024.104001
dc.identifier.urihttps://repositorio.univap.br/handle/123456789/402
dc.language.isoen_US
dc.publisherPhotodiagnosis and Photodynamic Therapy, Elsevier
dc.rights.holderLuciana Maria Cortez Marcolino
dc.rights.holderJéssica Aparecida Ribeiro Ambrosio
dc.rights.holderJuliana Guerra Pinto
dc.rights.holderIsabelle Ferreira
dc.rights.holderAndreza Ribeiro Simioni
dc.rights.holderJuliana Ferreira-Strixino
dc.subject.keywordLeishmania
dc.subject.keywordPhotodynamic therapy
dc.subject.keywordCurcumin
dc.subject.keywordNeglected Tropical Disease (NTDs)
dc.subject.keywordDrug delivery BSA nanocapsule
dc.titlePhotodynamic therapy of cationic and anionic BSA-curcumin nanoparticles on amastigotes of Leishmania braziliensis and Leishmania major and Leishmania amazonensis
dc.typeArtigos de Periódicos

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