Infrared Spectroscopy Based Study of Biochemical Changes in Saliva during Maximal Progressive Test in Athletes

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dc.contributor.authorVieira, Claudio Augusto Gonçalves de Araujo
dc.contributor.authorPupin, Breno
dc.contributor.authorBhattacharjee, Tanmoy T.
dc.contributor.authorSakane, Kumiko Koibuchi
dc.date.accessioned2025-06-13T12:11:29Z
dc.date.available2025-06-13T12:11:29Z
dc.date.issued22021
dc.description.abstractThis study aims to explore biochemical changes in saliva during cardiorespiratory exercise using attenuated-total- reflectance–Fourier-transform-infrared-spectroscopy (ATR-FTIR). Saliva and blood samples were obtained from six athletes at rest, and after running at speeds of 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, and 20 kilometers-per-hour (km/h) on a treadmill (maximal stress test). Saliva ATR-FTIR spectra were analyzed using deconvolution and multivariate analysis. Area-under-the-curve calculations suggest differential changes in glucose, lactate, protein, lipids, carbohydrate and phosphate content in saliva during the test. Increases in glucose and lactate levels with increasing speeds were verified by simultaneous measurement of blood glucose and lactate levels using standard equipment (Roche®). Multivariate principal-component-analysis (PCA) showed discrete clusters for low (rest-14 km/h) and high (15 – 20 km/h) speeds, and PCA–linear-discriminant-analysis showed 100% classification of 18 – 20 km/h as high speed. Overall, results suggest the possibility of using this non-invasive saliva-based ATR-FTIR method for biochemical assessment during sports exercise and stress tests.
dc.description.physical7 p.
dc.format.mimetypePDF
dc.identifier.affiliationUniversidade do Vale do Paraíba
dc.identifier.affiliationUniversity of Otago
dc.identifier.bibliographicCitationVIEIRA, C. A. G. A. et al. Infrared Spectroscopy Based Study of Biochemical Changes in Saliva during Maximal Progressive Test in Athletes. Analytical Sciences, v. 37, n. 8, p. 1157-1163, 2021. Disponível em: https://link.springer.com/10.2116/analsci.20P395.
dc.identifier.doi10.2116/analsci.20P395
dc.identifier.urihttps://repositorio.univap.br/handle/123456789/1001
dc.language.isoen_US
dc.publisherSpringer Nature Link
dc.rights.holderAnalytical Sciences
dc.subject.keywordMaximal stress test
dc.subject.keywordATR-FTIR
dc.subject.keywordPCA-LDA
dc.subject.keywordBiochemical change
dc.titleInfrared Spectroscopy Based Study of Biochemical Changes in Saliva during Maximal Progressive Test in Athletes
dc.typeArtigos de Periódicos

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