Photobiomodulation by LED 660 nm and Taurine against H2O2 oxidative stress in SH-SY5Y cells

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dc.contributor.authorRossato, Rafaella Carvalho
dc.contributor.authorSalles, Geisa Rodrigues
dc.contributor.authorAlbuquerque, Amanda Lira
dc.contributor.authorPorcionatto, Marimélia Aparecida
dc.contributor.authorGranato, Alessandro Eustáquio Campos
dc.contributor.authorUlrich, Henning
dc.contributor.authorSantos, Mariela Inês Batista dos
dc.contributor.authorSoares, Cristina Pacheco
dc.date.accessioned2025-08-26T12:19:10Z
dc.date.available2025-08-26T12:19:10Z
dc.date.issued22025
dc.description.abstractAlzheimer's Disease (AD) is a progressive uncurable neurodegenerative pathology affecting millions worldwide. Photo- biomodulation and Taurine are promising alternatives for preventing and reducing the rapid progression of neurodegenera- tion, stimulating the reconstructing of neural tissue structures, especially improving mitochondrial activity, which is highly impaired in AD. In this study, the mitochondrial effects of Taurine combined with light emitting diode (LED) irradiation were evaluated on human neuroblastoma cells (SH-SY5Y), under oxidative stress condition by hydrogen peroxide (H2O2) exposure, a considerable modulator in AD. We evaluated LED irradiation at the wavelength of 660 nm and Taurine under different concentrations before and together with exposing SH-SY5Y cells to different concentrations of H2O2, assessing mitochondrial activity by the MTT colorimetric test and labeling live cells mitochondria by the fluorescent probe MitoTracker. Cell viability was also evaluated by the trypan blue exclusion assay, and cellular morphological structures were imaged by scanning electron microscopy (SEM). Neuroprotective effects were achieved by both LED irradiation and LED irradia- tion + Taurine when cells were exposed to them before H2O2-induced stress. Comparing both agents, LED irradiation at 660 nm is sufficient to improve mitochondrial activity, however, healthy mitochondrial morphology was only observed when cells were treated with Taurine together with LED irradiation, representing affordable candidates that act in synergy against oxidative stress, one of the main contributors to neurodegeneration.
dc.description.physical11 p.
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipFinanciadora de Estudos e Projetos (Finep)
dc.description.uriFINEP ( 01.13.0275.00) CAPES (Code 001) FAPESP (2022/08664–4, 2016/17984–1, 2018/12605–8, 2018/07366–4 and 2012/50880–4) CNPq (406396/2021 and 308012/2021–6)
dc.format.mimetypePDF
dc.identifier.affiliationUniversidade do Vale do Paraíba
dc.identifier.affiliationUniversidade Federal de São Paulo
dc.identifier.affiliationUniversidade de São Paulo
dc.identifier.affiliationUniversidade Estadual Paulista
dc.identifier.bibliographicCitationROSSATO, R. C. et al. Photobiomodulation by LED 660 nm and Taurine against H2O2 oxidative stress in SH-SY5Y cells. Lasers in Medical Science, v. 40, n. 1, p. 1-11, 2025. Disponível em: 10.1007/s10103-025-04467-y.
dc.identifier.doi10.1007/s10103-025-04467-y
dc.identifier.urihttps://repositorio.univap.br/handle/123456789/1040
dc.language.isoen_US
dc.publisherSpringer Nature Link
dc.rights.holderLasers in Medical Science
dc.subject.keywordAlzheimer's Disease
dc.subject.keywordPhotobiomodulation
dc.subject.keywordTaurine
dc.subject.keywordOxidative stress
dc.titlePhotobiomodulation by LED 660 nm and Taurine against H2O2 oxidative stress in SH-SY5Y cells
dc.typeArtigos de Periódicos

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