Antimicrobial Effect of the Amniotic Membrane Isolated and Associated with Photodynamic Therapy

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dc.contributor.authorSantos, Amanda Cerquearo Rodrigues dos
dc.contributor.authorTeodoro, Guilherme Rodrigues
dc.contributor.authorFerreira-Strixino, Juliana
dc.contributor.authorSant’Anna, Luciana Barros
dc.date.accessioned2025-03-12T17:36:05Z
dc.date.available2025-03-12T17:36:05Z
dc.date.issued22023-03-08
dc.description.abstractMicrobial control through alternative therapies, such as the amniotic membrane (AM) and antimicrobial photodynamic therapy (aPDT), has been gaining prominence with the advancement of bacterial resistance to conventional treatments. This study aimed to evaluate the antimicrobial effect of AM isolated and associated with aPDT using the PHTALOX® as a photosensitizer (PS) against Staphylococcus aureus and Pseudomonas aeruginosa biofilms. The groups studied were: C+; L; AM; AM+L; AM+PHTX; and AM+aPDT. The irradiation parameters were 660 nm, 50 J.cm−2, and 30 mW.cm−2. Two independent microbiological experiments were carried out in triplicate, and the results were analyzed by CFU/mL counting and a metabolic activity test, both statistically analyzed (p < 0.05). The integrity of the AM was verified after the treatments by a scanning electron microscope (SEM). The groups AM, AM+PHTX, and, mainly, AM+aPDT showed a statistical difference When compared to C+ regarding the decrease in CFU/mL and metabolic activity. SEM analysis showed significant morphological alterations in the AM+PHTX and AM+aPDT groups. The treatments with AM isolated or associated with PHTALOX® were adequate. The association had potentiated the biofilm effect, and the morphological differences presented by AM after treatment did not hinder its antimicrobial effect, encouraging its use in biofilm formation locals.
dc.description.physical17 f.
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.uriCAPES (process number 88887.604760/2021-00/finance code 001) This study was financed by the Center for Studies and Microbiological Analysis (CEAM) by Golden Technology©
dc.format.mimetypepdf
dc.identifier.affiliationUniversidade do Vale do Paraíba
dc.identifier.affiliationUniversidade do Vale do Paraíba
dc.identifier.affiliationUniversidade do Vale do Paraíba
dc.identifier.affiliationUniversidade do Vale do Paraíba
dc.identifier.bibliographicCitationSANTOS, A. C. R. et al. Antimicrobial Effect of the Amniotic Membrane Isolated and Associated with Photodynamic Therapy. Journal of Functional Biomaterials, [S. l.], v. 14, n. 3, p. 151, 8 mar. 2023. Disponível em: https://www.mdpi.com/2079-4983/14/3/151.
dc.identifier.doi10.3390/jfb14030151
dc.identifier.urihttps://repositorio.univap.br/handle/123456789/685
dc.language.isoen_US
dc.publisherMDPI
dc.rights.holderMDPI
dc.rights.holderAmanda Cerquearo Rodrigues dos Santos
dc.rights.holderGuilherme Rodrigues Teodoro
dc.rights.holderJuliana Ferreira-Strixino
dc.rights.holderLuciana Barros Sant’Anna
dc.subject.keywordAmniotic membrane
dc.subject.keywordPhthalocyanine
dc.subject.keywordPhotosensitizer
dc.subject.keywordAntimicrobial effect
dc.subject.keywordPhotodynamic therapy
dc.titleAntimicrobial Effect of the Amniotic Membrane Isolated and Associated with Photodynamic Therapy
dc.typeArtigos de Periódicos

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