Evaluation of the Photodynamic Therapy with Curcumin on L. braziliensis and L. major Amastigotes

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dc.contributor.authorPereira, André Henrique Correia
dc.contributor.authorMarcolino, Luciana Maria Cortez
dc.contributor.authorPinto, Juliana Guerra
dc.contributor.authorStrixino, Juliana Ferreira
dc.date.accessioned2025-05-27T18:23:28Z
dc.date.available2025-05-27T18:23:28Z
dc.date.issued22021-05-25
dc.description.abstractCutaneous leishmaniasis (CL) is a neglected disease prevalent in tropical countries with the ability to cause skin lesions. Photodynamic therapy (PDT) represents a specific and topical option for the treatment of these lesions. This study evaluated the response of macrophages infected with L. braziliensis and L. major to PDT with curcumin. Curcumin concentrations were evaluated in serial dilutions from 500.0 to 7.8 μg/mL using LED (λ = 450 ± 5 nm), with a light dose of 10 J/cm2. The Trypan blue viability test, ultrastructural analysis by scanning electron microscopy (SEM), mitochondrial polarity by Rhodamine 123 (Rho 123), curcumin internalization by confocal microscopy, and counting of the recovered parasites after the PDT treatment were performed. The lowest concentrations of curcumin (15.6 and 7.8 μg/mL) presented photodynamic inactivation. Cell destruction and internalization of curcumin in both macrophages and intracellular parasites were observed in microscopy techniques. In addition, an increase in mitochondrial membrane polarity and a decrease in the number of parasites recovered was observed in the PDT groups. This study indicates that PDT with curcumin has the potential to inactivate infected macrophages and might act as a basis for future in vivo studies using the parameters herein discussed.
dc.description.physical14 p.
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.uriFAPESP: N. 2016/12211-4; CEPOF: grant number 2013/07276-1; FINEP: grant number 01.13.0275/00; CAPES: grant number Code 001.
dc.format.mimetypepdf
dc.identifier.affiliationUniversity of Vale do Paraíba
dc.identifier.affiliationUniversity of Vale do Paraíba
dc.identifier.affiliationUniversity of Vale do Paraíba
dc.identifier.affiliationUniversity of Vale do Paraíba
dc.identifier.bibliographicCitationPEREIRA, André Henrique Correia; MARCOLINO, Luciana Maria Cortez; PINTO, Juliana Guerra; FERREIRA-STRIXINO, Juliana. Evaluation of the Photodynamic Therapy with Curcumin on L. braziliensis and L. major Amastigotes. Antibiotics, [s. l.], v. 10, n. 6, p. 634, 25 maio 2021. DOI 10.3390/antibiotics10060634. Disponível em: https://www.mdpi.com/2079-6382/10/6/634.
dc.identifier.doihttps://doi.org/10.3390/antibiotics10060634
dc.identifier.urihttps://repositorio.univap.br/handle/123456789/974
dc.language.isoen
dc.publisherMDPI
dc.rights.holderMDPI
dc.rights.holderAndré Henrique Correia Pereira
dc.rights.holderLuciana Maria Cortez Marcolino
dc.rights.holderJuliana Guerra Pinto
dc.rights.holderJuliana Ferreira-Strixino
dc.subject.keywordPhotodynamic therapy
dc.subject.keywordCurcumin
dc.subject.keywordAmastigotes
dc.subject.keywordMacrophages
dc.subject.keywordCutaneous leishmaniasis
dc.titleEvaluation of the Photodynamic Therapy with Curcumin on L. braziliensis and L. major Amastigotes
dc.typeArtigos de Periódicos

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