Nano-Enabled Colorimetric Assay for the Detection of Paracoccidioides lutzii: Advancing Diagnostics with Nanotechnology

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dc.contributor.authorComparato Filho, Olavo de Osti
dc.contributor.authorCândido, Marcela Aparecida
dc.contributor.authorVentura, Aveline
dc.contributor.authorMorais, Flavia Villaça
dc.contributor.authorRaniero, Leandro José
dc.date.accessioned2026-01-15T17:53:07Z
dc.date.available2026-01-15T17:53:07Z
dc.date.issued22024
dc.description.abstractDeforestation is a common occurrence driven by agricultural expansion, urbanization, and infrastructure development. These activities often lead to increased human interaction with ecosys- tems, potentially exposing individuals to Paracoccidioides spores (P. brasiliensis and P. lutzii) found in the soil, resulting in Paracoccidioidomycosis (PCM). This fungal infection is endemic to specific regions in Latin America, such as Brazil, Colombia, Venezuela, and Argentina. Diagnosis typically involves a combination of clinical assessment, imaging techniques, and laboratory examinations. P. lutzii lacks the glycoprotein Gp43, a key antigenic protein utilized in serological tests for PCM diagnosis. In this study, a colorimetric test employing gold nanoparticles (AuNPs) and label-free methodology was employed for P. lutzii detection. The effectiveness of the label-free colorimetric test was assessed using a total of 100 samples. This detection was achieved through the amplification of the gp43 gene and the use of a specific probe (5′CAGGGGTGCG3′) in conjunction with AuNPs. The receiver operating characteristic curve was employed to assess the test, revealing that the method can accurately detect P. lutzii with a sensitivity of 100% and a specificity of 100%. The findings indicate a substantial impact on remote endemic regions attributable to the implementation of cost-effective diagnostic methodologies.
dc.description.physical9 p.
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.uriCNPq: 302158/2022-7
dc.format.mimetypePDF
dc.identifier.affiliationUniversidade do Vale do Paraíba
dc.identifier.bibliographicCitationCOMPARATO FILHO, O. O. et al. Nano-Enabled Colorimetric Assay for the Detection of Paracoccidioides lutzii: Advancing Diagnostics with Nanotechnology. Journal of Nanotheranostics, v. 5, n. 3, p. 75-83, 2024. Disponível em: https://www.mdpi.com/2624-845X/5/3/5.
dc.identifier.doi10.3390/jnt5030005
dc.identifier.urihttps://repositorio.univap.br/handle/123456789/1096
dc.language.isoen_US
dc.publisherMDPI
dc.rights.holderJournal of Nanotheranostics
dc.subject.keywordParacoccidioidomycosis
dc.subject.keywordP. lutzii
dc.subject.keywordLabel-free
dc.titleNano-Enabled Colorimetric Assay for the Detection of Paracoccidioides lutzii: Advancing Diagnostics with Nanotechnology
dc.typeArtigos de Periódicos

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