Enhanced viral inactivation by photodynamic therapy using curcumin and methylene blue: an in vitro study with bacteriophage as a viral mode

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dc.contributor.authorMiñán, Alejandro Guillermo
dc.contributor.authorInada, Natalia Mayumi
dc.contributor.authorFerreira-Strixino, Juliana
dc.contributor.authorMartins, Layla Farage
dc.contributor.authorSilva, Aline Maria da
dc.contributor.authorBagnato, Vanderlei Salvador
dc.date.accessioned2026-04-29T17:55:37Z
dc.date.available2026-04-29T17:55:37Z
dc.date.issued22027
dc.description.abstractThe global spread of SARS-CoV-2 highlighted the urgent need for broad-spectrum antiviral strategies. One promising approach is antimicrobial photodynamic therapy (aPDT), which, in the presence of oxygen, suitable photosensitizers and irradiation, generates reactive oxygen species capable of inactivating a broad spectrum of viruses. In this work, in vitro aPDT assays were performed using curcumin (Cur) and methylene blue (MB) as biocompatible photosensitizers, with bacteriophage ZC01 serving as the viral model and irradiation at 450 nm and 660 nm. Bacteriophages are frequently used as robust viral models due to their structural tolerance to photodynamic treatments compared with many mammalian viruses. Under the experimental conditions employed, a high viral load (B109 PFU mL-1) was used to represent a challenging inactivation scenario. The combination of curcumin and methylene blue at fluences of 12.5 and 25 J cm-2 per wavelength produced viral load reductions of 7.01log10 and 9.14log10, respectively. These results indicate that Cur/MB combination can achieve efficient photodynamic viral inactivation under moderate irradiation fluences. This in vitro platform may contribute to the development of photodynamic antiviral strategies and provides a practical system for evaluating potential photosensitizers and irradiation conditions for viral photoinactivation.
dc.description.physical8 p.
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.uriFAPESP: 2013/ 07276-1, 2014/50857-8, 2016/12211-4 CNPq: 465360/2014-9, 350140/2022-7
dc.format.mimetypePDF
dc.identifier.affiliationUniversidade do Vale do Paraíba
dc.identifier.affiliationUniversidad Nacional de La Plata
dc.identifier.affiliationUniversidade de São Paulo
dc.identifier.affiliationTexas A&M University
dc.identifier.bibliographicCitationMIÑÁN, Alejandro G. et al. Enhanced viral inactivation by photodynamic therapy using curcumin and methylene blue: an in vitro study with bacteriophage as a viral mode. New Journal of Chemistry, 2026. Disponível em: 10.1039/d5nj02994e.
dc.identifier.doi10.1039/d5nj02994e
dc.identifier.urihttps://repositorio.univap.br/handle/123456789/1181
dc.language.isoen_US
dc.publisherThe Royal Society of Chemistry
dc.rights.holderThe Royal Society of Chemistry
dc.subject.keywordPhotodynamic therapy
dc.subject.keywordMethylene blue
dc.titleEnhanced viral inactivation by photodynamic therapy using curcumin and methylene blue: an in vitro study with bacteriophage as a viral mode
dc.typeArtigos de Periódicos

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