Gymnema sylvestre as a Potential Anti-Inflammatory and Anti-Biofilm Agent Against Anaerobic Infections: An In Vitro Study

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dc.contributor.authorMiranda, Diego Garcia
dc.contributor.authorTome, Fernanda Malagutti
dc.contributor.authorMiguel, Manuela Maria Viana
dc.contributor.authorLiberato, Sabrina Ferreira dos Santos
dc.contributor.authorMarcucci, Maria Cristina
dc.contributor.authorVigerelli, Hugo
dc.contributor.authorRodrigues, Flavia Pires
dc.contributor.authorSoares, Cristina Pacheco
dc.contributor.authorGodoi, Bruno Henrique
dc.contributor.authorCarrouel, Florence
dc.contributor.authorOliveira, Luciane Dias de
dc.contributor.authorRamos, Lucas de Paula
dc.date.accessioned2025-08-25T14:19:16Z
dc.date.available2025-08-25T14:19:16Z
dc.date.issued22025
dc.description.abstractThis study evaluates the antimicrobial activity of the glycolic extract of G. sylvestre against anaerobic pathogens, along with its cytotoxicity, genotoxicity, anti-inflammatory activity, antioxidant effects, and phytochemical composition. Phytochemical analysis was conducted using high-performance liquid chromatography and liquid chromatography– mass spectrometry, while the antioxidant effect was assessed through a DPPH assay. An- timicrobial action was tested on planktonic cultures and biofilms of Porphyromonas gingivalis, Porphyromonas endodontalis, Parvimonas micra, and Fusobacterium nucleatum. Cytotoxicity was evaluated using mouse macrophages (RAW 264.7), rat fibroblasts (L929), and human keratinocytes (HaCaT). Anti-inflammatory effects were measured by an immunoenzymatic assay (ELISA) on RAW 264.7 cells. Statistical analysis was performed using a one-way ANOVA and Tukey’s test. Phytochemical analysis revealed the presence of phenolic com- pounds and flavonoids. The extract demonstrated a reduction of over 95% in biofilms of P. gingivalis, P. micra, and F. nucleatum within 5 min of treatment. Cell viability (HaCaT) remained above 80%. Antioxidant activity showed an EC50 of 353.43 μg/mL, achieving a 50% reduction in free radicals. A significant decrease in TNF-α (a pro-inflammatory cytokine) and an increase in IL-10 (an anti-inflammatory cytokine) were observed. In conclusion, the extract of G. sylvestre exhibits promising potential as a therapeutic agent for treating anaerobic infections, inflammation, and oxidative stress.
dc.description.physical17 p.
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipFinanciadora de Estudos e Projetos (Finep)
dc.description.uriCAPES (88887.891487/2023-00 and 88887.829787/2023-00) Finep (01.12.0450.00 and 01.09.0278-00)
dc.format.mimetypePDF
dc.identifier.affiliationUniversidade do Vale do Paraíba
dc.identifier.affiliationUniversidade do Estado de São Paulo
dc.identifier.affiliationWoman-Mother-Child Hospital
dc.identifier.affiliationUniversity of Lyon
dc.identifier.affiliationUniversidade Paulista
dc.identifier.affiliationInstituto Butantan
dc.identifier.affiliationUniversity of Leeds
dc.identifier.affiliationUniversidade Federal de Alfenas
dc.identifier.bibliographicCitationMIRANDA, D. G. et al. Gymnema sylvestre as a Potential Anti-Inflammatory and Anti-Biofilm Agent Against Anaerobic Infections: an In Vitro Study. Plants-Basel, v. 14, n. 4, p. 1-17, 2025. Disponível em: 10.3390/plants14040497.
dc.identifier.doi10.3390/plants14040497
dc.identifier.urihttps://repositorio.univap.br/handle/123456789/1035
dc.language.isoen_US
dc.publisherMDPI
dc.rights.holderPlants-Basel
dc.subject.keywordAntimicrobial agents
dc.subject.keywordAnti-inflammatory agents
dc.subject.keywordBiofilms
dc.subject.keywordGram-negative anaerobic cocci
dc.titleGymnema sylvestre as a Potential Anti-Inflammatory and Anti-Biofilm Agent Against Anaerobic Infections: An In Vitro Study
dc.typeArtigos de Periódicos

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