In vitro and in vivo evaluation of rotary-jet-spun poly(ɛ- caprolactone) with high loading of nano-hydroxyapatite

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dc.contributor.authorAndrade, Telmo M.
dc.contributor.authorMello, Daphne de Camargo Reis
dc.contributor.authorElias, Conceição de Maria Vaz
dc.contributor.authorAbdala, Julia Marinzeck de Alcantara
dc.contributor.authorSilva, Edmundo
dc.contributor.authorVasconcellos, Luana Marotta Reis de
dc.contributor.authorTim, Carla Roberta
dc.contributor.authorMarciano, Fernanda Roberta
dc.contributor.authorLobo, Anderson Oliveira
dc.date.accessioned2026-02-09T20:17:43Z
dc.date.available2026-02-09T20:17:43Z
dc.date.issued22019
dc.description.abstractHerein, poly(ɛ-caprolactone) (PCL) mats with different amounts of nanohydroxyapatite (nHAp) were produced using rotary- jet spinning (RJS) and evaluated in vitro and in vivo. The mean fiber diameters of the PCL, PCL/nHAp (3%), PCL/nHAp (5%), and PCL/nHAp (20%) scaffolds were 1847±1039, 1817±1044, 1294±4274, and 845±248nm, respectively. Initially, all the scaffolds showed superhydrophobic behavior (contact angle around of 140oC), but decreased to 80° after 30 min. All the produced scaffolds were bioactive after soaking in simulated body fluid, especially PCL/nHAp (20%). The crystallinity of the PCL scaffolds decreased progressively from 46 to 21% after incorporation of 20% nHAp. In vitro and in vivo cytotoxicity were investigated, as well as the mats’ ability to reduce bacteria biofilm formation. In vitro cellular differentiation was evaluated by measuring alkaline phosphatase activity and mineralized nodule formation. Overall, we identified the total ideal amount of nHAp to incorporate in PCL mats, which did not show in vitro or in vivo cytotoxicity and promoted lamellar bone formation independently of the amounts of nHAp. The scaffolds with nHAp showed reduced bacterial proliferation. Alizarin red staining was higher in materials associated with nHAp than in those without nHAp. Overall, this study demonstrates that PCL with nHAp prepared by RJS merits further evaluation for orthopedic applications
dc.description.physical14 p.
dc.format.mimetypePDF
dc.identifier.affiliationUniversidade do Vale do Paraíba
dc.identifier.affiliationUniversidade Brasil
dc.identifier.affiliationUniversidade Estadual de São Paulo
dc.identifier.bibliographicCitationANDRADE, T. M. et al. In vitro and in vivo evaluation of rotary-jet-spun poly(ɛ- caprolactone) with high loading of nano-hydroxyapatite. Journal of Materials Science: Materials in Medicine, v. 30, n. 19, p. 1-14, 2019. Disponível em: https://doi.org/10.1007/s10856-019-6222-1.
dc.identifier.doi10.1007/s10856-019-6222-1
dc.identifier.urihttps://repositorio.univap.br/handle/123456789/1141
dc.language.isoen_US
dc.publisherSpringer
dc.rights.holderJournal of Materials Science: Materials in Medicine
dc.subject.keywordBioinspired Materials
dc.subject.keywordBiomedical Materials
dc.subject.keywordBiomaterials
dc.subject.keywordPolyhydroxyalkanoates
dc.subject.keywordSoft Materials
dc.titleIn vitro and in vivo evaluation of rotary-jet-spun poly(ɛ- caprolactone) with high loading of nano-hydroxyapatite
dc.typeArtigos de Periódicos

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