Continuous production of enzymes under carbon-limited conditions by Trichoderma harzianum P49P11

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dc.contributor.authorGelain, Lucas
dc.contributor.authorKingma, Esther
dc.contributor.authorPradella, José Geraldo da Cruz
dc.contributor.authorCosta, Aline Carvalho da
dc.contributor.authorWielen, Luuk Van Der
dc.contributor.authorGulik, Walter Martin van
dc.date.accessioned2025-04-09T14:31:18Z
dc.date.available2025-04-09T14:31:18Z
dc.date.issued22021
dc.description.abstractCarbon-limited chemostat cultures were performed using different carbon sources (glucose, 10 and 20 g/L; sucrose, 10 g/L; fructose/glucose, 5.26/5.26 g/L; carboxymethyl cellulose, 10 g/L; and carboxymethyl cellulose/glucose, 5/5 g/L) to verify the capability of the wild type strain Trichoderma harzianum to produce extracellular enzymes. All chemostat cultures were carried out at a fixed dilution rate of 0.05 h . Experiments using glucose, fructose/glucose and sucrose were performed in duplicate. Glucose condition was found to induce the production of enzymes that can catalyse the hydrolysis of p-nitro- phenyl-b-D-glucopyranoside (PNPGase). A concentration of 20 g/L of glucose in the feed provided the highest productivity (1048 ± 16 U/mol h). Extracellular polysaccharides were considered the source of inducers. Based on the obtained results, a new PNPGase production process was developed using mainly glucose. This process raises interesting possibilities of synthesizing the inducer substrate and the induced enzymes in a single step using an easily assimilated carbon source under carbon-limited conditions.
dc.description.physical7 p.
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.uriFAPESP ( 2014/22537–9)
dc.format.mimetypePDF
dc.identifier.affiliationUniversidade do Vale do Paraíba
dc.identifier.affiliationDelft University of Technology
dc.identifier.affiliationUniversity of Limerick
dc.identifier.affiliationUniversidade Federal de São Paulo
dc.identifier.bibliographicCitationGELAIN, Lucas et al. Continuous production of enzymes under carbon-limited conditions by Trichoderma harzianum P49P11. Fungal Biology, v. 125, v. 3, p. 177-183, 2021. Disponível em: https://linkinghub.elsevier.com/retrieve/pii/S1878614620301604.
dc.identifier.doi10.1016/j.funbio.2020.10.008
dc.identifier.urihttps://repositorio.univap.br/handle/123456789/831
dc.language.isoen_US
dc.publisherElsevier
dc.rights.holderFungal Biology
dc.subject.keywordEnzyme production
dc.subject.keywordContinuous fermentation process
dc.subject.keywordCarbon limitation
dc.subject.keywordPNPGase
dc.subject.keywordGlucose
dc.subject.keywordExtracellular polysaccharides
dc.titleContinuous production of enzymes under carbon-limited conditions by Trichoderma harzianum P49P11
dc.typeArtigos de Periódicos

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