Efficiency of Antimicrobial Photodynamic Therapy with Photodithazine® on MSSA and MRSA Strains

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dc.contributor.authorSouza, Beatriz Müller Nunes
dc.contributor.authorPinto, Juliana Guerra
dc.contributor.authorPereira, André Henrique Correia
dc.contributor.authorMiñán, Alejandro Guillermo
dc.contributor.authorStrixino, Juliana Ferreira
dc.date.accessioned2025-06-09T19:12:13Z
dc.date.available2025-06-09T19:12:13Z
dc.date.issued22021-07-17
dc.description.abstractStaphylococccus aureus is a ubiquitous and opportunistic bacteria associated with high mortality rates. Antimicrobial photodynamic therapy (aPDT) is based on the application of a light source and a photosensitizer that can interact with molecular oxygen, forming Reactive Oxygen Species (ROS) that result in bacterial inactivation. This study aimed to analyze, in vitro, the action of aPDT with Photodithazine® (PDZ) in methicillin-sensitive Staphylococcus aureus (MSSA) and methicillin-resistant Staphylococcus aureus (MRSA) strains. The strains were incubated with PDZ at 25, 50, 75, and 100 mg/L for 15 min and irradiated with fluences of 25, 50, and 100 J/cm2. The internalization of PDZ was evaluated by confocal microscopy, the bacterial growth by counting the number of colony-forming units, as well as the bacterial metabolic activity post-aPDT and the production of ROS. In both strains, the photosensitizer was internalized; the production of ROS increased when the aPDT was applied; there was a bacterial reduction compared to the control at all the evaluated fluences and concentrations; and, in most parameters, it was obtained complete inactivation with significant difference (p < 0.05). The implementation of aPDT with PDZ in clinical strains of S. aureus has resulted in its complete inactivation, including the MRSA strains.
dc.description.physical17 p.
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipFinanciadora de Estudos e Projetos (Finep)
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.uriFAPESP: 2016/12211-4; CEPOF: 2013/07276-1; FINEP: 01.13.0275/00; CAPES: Finance Code 001.
dc.format.mimetypepdf
dc.identifier.affiliationUniversity of Vale do Paraíba
dc.identifier.affiliationUniversity of Vale do Paraíba
dc.identifier.affiliationUniversity of Vale do Paraíba
dc.identifier.affiliationUniversidad Nacional de La Plata
dc.identifier.affiliationUniversity of Vale do Paraíba
dc.identifier.bibliographicCitationSouza, B.M.N.; Pinto, J.G.; Pereira, A.H.C.; Miñán, A.G.; Ferreira-Strixino, J. Efficiency of Antimicrobial Photodynamic Therapy with Photodithazine® on MSSA and MRSA Strains. Antibiotics 2021, 10, 869. https://doi.org/10.3390/ antibiotics10070869
dc.identifier.doi<http://doi.org/10.3390/antibiotics10070869>
dc.identifier.urihttps://repositorio.univap.br/handle/123456789/983
dc.language.isoen
dc.publisherMDPI
dc.rights.holderBeatriz Müller Nunes Souza
dc.rights.holderJuliana Guerra Pinto
dc.rights.holderAndré Henrique Correia Pereira
dc.rights.holderAlejandro Guillermo Miñán
dc.rights.holderJuliana Ferreira-Strixino
dc.subject.keywordAntimicrobial photodynamic therapy
dc.subject.keywordMRSA
dc.subject.keywordMSSA
dc.subject.keywordPhotodithazine®
dc.titleEfficiency of Antimicrobial Photodynamic Therapy with Photodithazine® on MSSA and MRSA Strains
dc.typeArtigos de Periódicos

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