Chlorin e6-EGF conjugated gold nanoparticles as a nanomedicine based therapeutic agent for triple negative breast cancer

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dc.contributor.authorCastilho, Maiara Lima
dc.contributor.authorJesus, Viviane Paula dos Santos
dc.contributor.authorVieira, Paula Fonseca Antunes
dc.contributor.authorHewitt, Kevin Cecil
dc.contributor.authorRaniero, Leandro José
dc.date.accessioned2025-06-12T15:17:55Z
dc.date.available2025-06-12T15:17:55Z
dc.date.issued22021
dc.description.abstractTo develop a treatment modality for triple-negative breast cancer, we investigated the efficacy of a bifunctional theranostic nanoprobes (BN) during Photodynamic Therapy (PDT) on human breast carcinoma and normal human cells. The BN is a 21 nm gold nanoparticles functionalized with Chlorin e6 (Ce6) and Epidermal Growth Factor (EGF). Attachment to gold nanoparticle stabilizes Ce6 while EGF acts as a cancer cell targeting agent. Fluorescence Spectroscopy and Confocal Fluorescence Microscopy revealed a gradual uptake of nanoprobes into cancer cells at an average rate of 63 BN/min. Cell viability assays showed that 0.2 μg/mL BN concentration was highly cytotoxic to cancer cells (86 %), but not normal cells. At this concentration, 58 % cancer cells were necrotic and 38 % apoptotic, while the reactive oxygen species (ROS) was 9-fold higher in cancer cells compared to normal. Overall, results suggest that BN mediated PDT can achieve targeted cancer cell death with high efficiency.
dc.description.physical7 p.
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.uriFAPESP (Project 2017/07519-2) CNPq (Project 302944/2018-4)
dc.format.mimetypePDF
dc.identifier.affiliationUniversidade do Vale do Paraíba
dc.identifier.affiliationDalhousie University
dc.identifier.bibliographicCitationCASTILHO, M. L. et al. Chlorin e6-EGF conjugated gold nanoparticles as a nanomedicine based therapeutic agent for triple negative breast cancer. Photodiagnosis and Photodynamic Therapy, v. 33, p. 1-7, 2021. Disponível em:
dc.identifier.doi10.1016/j.pdpdt.2021.102186
dc.identifier.urihttps://repositorio.univap.br/handle/123456789/995
dc.language.isoen_US
dc.publisherElsevier
dc.rights.holderPhotodiagnosis and Photodynamic Therapy
dc.subject.keywordBifunctional nanoprobes
dc.subject.keywordEpidermal growth factor
dc.subject.keywordChlorin e6
dc.subject.keywordTriple-negative breast cancer
dc.subject.keywordTheranostic
dc.subject.keywordPhotodynamic Therapy
dc.titleChlorin e6-EGF conjugated gold nanoparticles as a nanomedicine based therapeutic agent for triple negative breast cancer
dc.typeArtigos de Periódicos

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