Antibacterial activity of silver nanoparticles functionalized with amikacin applied against multidrug-resistant acinetobacter baumannii

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dc.contributor.authorCamargo, Larissa de Oliveira
dc.contributor.authorFontoura, Inglid
dc.contributor.authorVeriato, Thaís da Silva
dc.contributor.authorRaniero, Leandro José
dc.contributor.authorCastilho, Maiara Lima
dc.date.accessioned2025-03-31T20:56:47Z
dc.date.available2025-03-31T20:56:47Z
dc.date.issued22023
dc.description.abstractBackground: Multidrug-resistant bacteria are one of the world's biggest health problems; therefore, improving the spectrum of action of antibiotics could be necessary to reverse this situation. Amikacin and silver salts have well-known antimicrobial properties. However, both drugs lost their effectiveness against some bacteria, such as Acinetobacter baumannii. This work aims to develop a nanodrug from silver nanoparticles (AgNPs) functionalized with Amikacin against multidrug-resistant Acinetobacter baumannii. Methods: AgNPs were produced using the bottom-up methodology and functionalized with Amikacin modified by the carbodiimide-based chemistry, forming AgNPs@Amikacin. Susceptibility tests were performed using Amikacin-resistant Acinetobacter baumannii strains to assess the bacteriostatic and bactericidal potential of the developed nanodrug. The clinical strains were induced to form a biofilm, and biomass quantification and the metabolic activity were determined. Results: The AgNPs have a hydrodynamic diameter of the particles with a bimodal distribution, with a size of 37.84 nm. The FT-IR spectrum of AgNPs@Amikacin exhibits vibrational modes corresponding to Amikacin, confirming the conjugation to AgNPs. Susceptibility testing demonstrated a minimal inhibitory and bactericidal concentration of < 0.5 µg/mL. The AgNPs@Amikacin reduced the biofilm metabolic activity of Acinetobacter baumannii at rates ≥ 50%, characterized by the minimal biofilm inhibition concentrations. Conclusions: Results demonstrate a promising development of a new nanodrug with lower concentrations, less toxicity, and greater efficacy against multidrug-resistant Acinetobacter baumannii.
dc.description.physical8 p.
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.uriFAPESP: Project n. 2018/23898-6); CNPq Projects n. 427602/2018-1; 302994/2018-4.
dc.format.mimetypepdf
dc.identifier.affiliationUniversity of Paraiba Valley
dc.identifier.affiliationUniversity of Paraiba Valley
dc.identifier.affiliationUniversity of Paraiba Valley
dc.identifier.affiliationUniversity of Paraiba Valley
dc.identifier.affiliationUniversity of Paraiba Valley,
dc.identifier.bibliographicCitationCAMARGO, L. D. O. et al. Antibacterial activity of silver nanoparticles functionalized with amikacin applied against multidrug-resistant acinetobacter baumannii. American Journal of Infection Control, [S. l.], v. 51, n. 8, p. 871–878, ago. 2023. Disponível em: https://linkinghub.elsevier.com/retrieve/pii/S019665532200880X.
dc.identifier.doihttps://doi.org/10.1016/j.ajic.2022.12.009
dc.identifier.urihttps://repositorio.univap.br/handle/123456789/785
dc.language.isoen_US
dc.publisherElsevier
dc.rights.holderAssociation for Professionals in Infection Control and Epidemiology
dc.subject.keywordNanopartículas de prata
dc.subject.keywordSilver nanoparticles
dc.subject.keywordMultidrug-resistant
dc.subject.keywordAcinetobacter baumannii
dc.subject.keywordAmikacin Aminoglycoside
dc.titleAntibacterial activity of silver nanoparticles functionalized with amikacin applied against multidrug-resistant acinetobacter baumannii
dc.typeArtigos de Periódicos

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