Effects of Preservation Methods in the Composition of the Placental and Reflected Regions of the Human Amniotic Membrane

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dc.contributor.authorMoraes, Jéssica Tereza Guedes de Oliveira
dc.contributor.authorCosta, Maíra Maftoum
dc.contributor.authorAlves, Paula Cristina Santos
dc.contributor.authorSant’Anna, Luciana Barros
dc.date.accessioned2025-06-12T18:41:19Z
dc.date.available2025-06-12T18:41:19Z
dc.date.issued22021
dc.description.abstractThe human amniotic membrane (AM) is emerging as an in- teresting biomaterial for regenerative medicine due to its biological and mechanical proprieties. The beneficial effects of the AM are probably related to its bioactive factors pro- duced by local cells and stored in the stromal matrix. How- ever, the search for a preservation method capable of pre- serving AM properties remains a challenge. The aim of this study was to evaluate important features of 2 anatomical re- gions of the human AM (reflected and placental amnion) af- ter different preservation methods. For this purpose, human placentas were harvested and processed for AM isolation and storage at 2 different conditions: room temperature for 18 h in DMEM (fresh AM) and −80°C in DMEM/glycerol solu- tion for 30 days (cryopreserved AM). After the storage peri- od, the structural integrity of the membrane was assessed by histological and Picrosirius polarization analysis, cellular vi- ability analysis was performed using the MTT assay, and the soluble proteins were quantified with the Qubit Protein Assay Kit. Both preservation protocols reduced the cell viabili- ty, mainly in the placental amnion region of the AM, but pre- served the morphology of epithelial and stromal layers, as well as the organization and distribution of collagen fibers. There was a reduction in soluble proteins only in fresh AM. Importantly, the cryopreserved AM group presented the same concentration as the control group. In conclusion, the cryopreservation using DMEM/glycerol was ideal for pre- serving the structural integrity and soluble protein content, indicating the feasibility of this method in preserving AM for its use in regenerative medicine.
dc.description.physical11 p.
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.uriFAPESP (2016/10827-8)
dc.format.mimetypePDF
dc.identifier.affiliationUniversidade do Vale do Paraíba
dc.identifier.bibliographicCitationMORAES, J. T. G. O. et al. Effects of Preservation Methods in the Composition of the Placental and Reflected Regions of the Human Amniotic Membrane. Cells Tissues Organs, v. 210, n. 1, p. 66-76, 2021. Disponível em: https://karger.com/CTO/article/doi/10.1159/000515448.
dc.identifier.doi10.1159/000515448
dc.identifier.urihttps://repositorio.univap.br/handle/123456789/999
dc.language.isoen_US
dc.publisherKarger
dc.rights.holderCells Tissues Organs
dc.subject.keywordAmniotic membrane
dc.subject.keywordPlacental amnion
dc.subject.keywordPicrosirius
dc.subject.keywordReflected amnion
dc.titleEffects of Preservation Methods in the Composition of the Placental and Reflected Regions of the Human Amniotic Membrane
dc.typeArtigos de Periódicos

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