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Item Photobiomodulation applied in post-surgical female-to-male surgery: case report(Erciyes Üniversitesi) Arisawa, Emilia Angela Lo Schiavo; Martins, Rodrigo Álvaro Lopes; Martins, Patrícia Sardinha Leonardo Lopes; Meneguzzo, Daiane Thais; Arisawa, Ana Amélia Lo Schiavo; Gallagher, SidhbhPhotobiomodulation (PBM) is an emerging medical technology with therapeutic effects that reduce or inhibit inflammatory mediators and cytokines, decreasing pain and local edema. However, the number of papers reporting the application of PBM in the post-surgical period is still scarce. This case report aims to evaluate the action of PBM applied after bilateral mastectomy with free nipple grafts regarding collateral effects such as pain, edema and hematoma. Patients underwent breast surgery with free nipples grafts. PBM sessions were applied 5, 24, 48 and 72 hours after surgery using a diode laser Platinum 4 equipment with four wavelengths (650, 810, 915 and 980 nm). Total energy applied was 1080 Joules distributed in six minutes of laser application over lymph node areas and the suture region. Both patients (A and B) related pain relief after PBM sessions (5, 24, 48 and 72 hours) after surgery. Also nipple sensitivity and overflow of reddish-color were reduced after 72h. The application of PBM reduced pain intensity and nipples hypersensitivity, suggesting reduction of inflammatory process. The benefits reported in this study suggest the indication of this complementary therapy to cosmetic procedures for transgender people.Item Non-thermal plasma applied to treating diabetic foot(CDRR Editors) Arisawa, Emilia Angela Lo Schiavo; Cardoso, Juliana Cunha; Vieira, Lucia; Oliveira, Rauirys Alencarde deObjective: Evaluate the effects of non-thermal plasma therapy (NTP) for the treatment of diabetic foot injuries in two patients, considering the evolution of the steps of the healing process, pain sensitivity, and quality of life. Methodology: Lesions in the lower limbs of two patients with Diabetes mellitus (DM) with similar anatomical location and dimensions were treated, one patient with controlled DM and the other classified as uncontrolled DM. The application of NTP was performed for 10 minutes, 3 times a week in direct contact with the lesions. The same protocol was applied in both clinical cases to allow a reliable evaluation of the healing process. Results and Discussion: The lesions presented progressive regression until their complete regeneration (100%), without the development of infections during treatment. Conclusion: The results obtained in the two clinical cases allow us to attest that the NTP application in the treatment of diabetic foot injuries resulting from DM constituted an important therapeutic tool for the evolution of the healing process of these injuries, with a significant reduction in the lesion area in a short period of time. The definition of the ideal treatment protocol depends on the expansion of the number of studies and the increase of the studied population.Item Intervenção de musicoterapia nos sinais vitais de crianças com transtorno do espectro autista: Estudo piloto(CDRR Editors) Magalhães, Juliana Macêdo; Batista, Pedro Venicius de Sousa; Arisawa, Emilia Angela Lo SchiavoObjetivo: Avaliar os sinais vitais de crianças com transtorno do espectro autista no contexto de intervenção de musicoterapia. Métodos: Estudo piloto experimental de intervenção de musicoterapia, desenvolvido no nordeste do Brasil. Sete crianças com transtorno do espectro autista foram avaliadas, distribuída em três para intervenção de musicoterapia e quatro sem intervenção. Os dados foram analisados utilizando testes não paramétricos. Resultados: As crianças apresentavam média de idade de oito anos, com maioria do sexo masculino. Descritivamente houve redução nos sinais vitais de 3,7% na saturação de O2, diminuição de 3,3 mmHg na pressão arterial sistólica e aumento de 5,0 bpm na frequência cardíaca no grupo que passou por intervenção de musicoterapia. O grupo sem intervenção apresentou aumento médio de 0,5% na saturação de O2 e de 2,5 mmHg na pressão arterial diastólica. Conclusão: Conclui-se que os efeitos da musicoterapia nos sinais vitais foram indicativos de respostas satisfatórias para as crianças com autismo.Item Fotobiomodulação no tratamento de neuralgia pós-herpética: caso clínico(Revista Univap) Silva, Erick Giovanni Reis; Arisawa, Emilia Angela Lo Schiavo; Martins, Rodrigo Alvaro Brandão Lopes; Gomes, Raimundo Nonato Silva; Nicolau, Renata Amadei; Pinto, Ana PaulaO presente caso clínico teve por objetivo avaliar a ação da fotobiomodulação (PBM) no tratamento de paciente com diagnóstico de Neuropatia Pós-Herpética (NPH), visando o alívio da dor e a melhora da qualidade de vida. Para a PBM utilizou-se um cluster com três lasers de GaAlAs, com os parâmetros: 660 nm, 100 mW, irradiação pontual, 40 s/ponto, 4 J/cm2 por ponto e total de 10 sessões. Para aferição da dor foram utilizadas a Escala Visual Analógica da Dor (EVA) e Algometria, e a qualidade de vida, com aplicação do Questionário de Avaliação da Qualidade de Vida -SF-36. Os dados obtidos foram submetidos à análise descritiva e ao teste estatístico T pareado, com nível de significância de 5% (p<0,05). Os resultados deste estudo demonstraram redução da dor quando comparadas a 1ª à 10ª sessão, tanto pela EVA quanto pela Algometria. Com relação à análise da qualidade de vida, foi observada uma melhora significativa em todos os oito domínios avaliados. Portanto, conclui-se que a PBM foi eficaz no tratamento da NPH visto que houve redução da dor e melhora na qualidade de vida. Esses resultados são importantes e podem nortear novas pesquisas clínicas que objetivem o emprego da PBM no tratamento da NPH.Item Feridas em pés diabéticos tratadas com membrana amniótica humana e terapia a laser de baixa intensidade: um estudo clínico piloto(HMP) Santos, Joelita de Alencar Fonseca; Nicolau, Renata Amadei; Sant’Anna, Luciana Barros; Paterno, Josne Carla; Cristovam, Priscila Cardoso; Gomes,, Jose Álvaro Pereira; Santos, José Diego Marques; Arisawa, Emilia Angela Lo SchiavoCONTEXTO: A terapia a laser de baixa intensidade (LLLT) e a aplicação de membrana amniótica humana (HAM) demonstraram ser opções viáveis para uso na cicatrização de feridas. OBJETIVO: Este estudo buscou comparar LLLT e HAM a um tratamento controle (hidrogel, solução salina e gaze) em pessoas com diabetes mellitus (DM) e úlceras nos pés. MÉTODOS: Usando um delineamento de estudo clínico piloto prospectivo, foram recrutados pacientes que receberam atendimento em um centro de saúde especializado no tratamento de feridas nos pés diabéticos entre novembro de 2016 e agosto de 2017. Os pacientes elegíveis deveriam ter entre 30 e 59 anos de idade; diagnóstico de DM tipo 2 (níveis de glicemia capilar pós-prandial entre 140 e 350 mg/dL); e ter úlceras nos pés em estágio 2 ou 3, não infectadas e em granulação, medindo menos de 7 cm por 3 cm. Pacientes imunossuprimidos e desnutridos ou aqueles com neoplasias ou em estado crítico não eram elegíveis para participar. Os pacientes receberam o tratamento controle (2 mg de hidrogel, solução salina e gaze), HAM (adesivos de HAM descongelado, aplicados com bordas sobrepostas) ou LLLT (sessão de fototerapia, 2 mg de hidrogel, solução salina e gaze) por 28 dias. Variáveis, medidas da área da ferida, pontuações da Pressure Ulcer Scale for Healing (PUSH) e pontuações da Visual Analog Scale (VAS) foram usadas para avaliar o progresso da melhora da ferida e a dor nos dias 7, 14, 21 e 28. Estatísticas descritivas foram usadas para analisar os perfis antropométricos e clínicos dos participantes. O teste de Kolmogorov-Smirnov foi usado para analisar a distribuição da amostra. O teste de Kruskal-Wallis com pós-teste de Dunn foi usado para avaliar as diferenças nas pontuações PUSH e VAS e no tamanho da ferida para análise intergrupo, e o teste U de Mann-Whitney foi usado para os mesmos resultados na análise intragrupo. O nível de significância foi de 5% ( P < 0,05). RESULTADOS: Vinte e sete (27) pacientes participaram (idade média, 51,4 anos; índice de massa corporal médio, 26,5 kg/m 2 ), com 9 pacientes em cada grupo de tratamento. Não foram observadas diferenças estatisticamente significativas nas variáveis clínicas ou antropométricas entre os grupos, mas as áreas médias basais das feridas foram diferentes (2,6 cm2 para o controle, 1,9 cm2 para o LLLT e 5,5 cm2 para os grupos HAM). As comparações intragrupo mostraram uma redução significativa na pontuação PUSH no grupo LLT entre os dias 0 e 21 (8,2 vs 4,9; P < 0,01) e dias 21 a 28 (4,9 vs 3,2; P < 0,001). Em todos os grupos de tratamento, a redução percentual foi significativamente diferente entre os dias 7 e 28. Nenhum resultado foi significativamente diferente entre os grupos. CONCLUSÃO: A área da ferida da úlcera do pé diabético, bem como os escores PUSH e VAS, apresentaram maior melhora em pacientes com DM que receberam LLLT ou HAM do que no grupo controle, mas as diferenças não foram significativas. Estudos maiores são necessários para comparar essas modalidades de tratamento.Item Saliva FTIR Spectra and Machine Learning for Autism Spectrum Disorder Diagnosis-Preliminary Study(IEEE) Pinto, Mayara Moniz Vieira; Arisawa, Emilia Angela Lo Schiavo; Raniero, Leandro José; Bhattacharjee, TanmoyThe diagnosis of Autism Spectrum Disorder (ASD) remains a challenge due to the lack of specific tests and biological markers. ASD is a neurodevelopmental disorder that affects in- dividuals throughout their lives, and its diagnosis allows access to treatments that improve their prognosis. Saliva analysis by Fourier Transform Infrared Spectroscopy (FTIR), which was not previ- ously reported, appears to be a promising diagnostic tool for ASD. This study acquired spectra from samples of 19 ASD and 19 control children. Spectral signatures suggest the dominance of protein secondary structures, β-pleated sheet and α-helix structures in ASD and control children, respectively. Support Vector Machine (SVM) gave the best diagnosis, with sensitivity, precision, and specificity being 92%, 94%, and 95%, respectively. Shapley values analysis to understand the impact of spectral features on the SVM classifier identified β-pleated and β-turn sheets as responsible for classification. Results indicate the potential of saliva-based FTIR for autism diagnosis, warranting a large-scale trial.Item Kinematic and sensory-motor analysis of the effects of treatments with photobiomodulation in rats with experimentally induced spinal injury(Springer Nature Link) Beloni, Luís Filipe Karatanasov; Lima, Leonardo Borges de; Correia, Débora Campos Chaves; Mendes, Alessandro Corrêa; Sannt'Anna, Luciana Barros; Arisawa, Emilia Angela Lo Schiavo; Lima, Fernanda Pupio Silva; Lima, Mario OliveiraThe aim of this study was to evaluate the kinematic and sensorimotor parameters after application of photobiomodula- tion (PBM) in rats with experimentally induced spinal cord injuries (SCI). The animals were randomly divided into 3 experimental groups containing 5 animals each and respecting the following arrangement: C (control, simulation of sur- gical procedures); I (injury, induction of SCI) and PBM (photobiomodulation, SCI+application of photobiomodulation therapy). All animals underwent movement tests after 3, 7, 10 and 14 days, respectively, after the surgical procedure, and the following movement parameters were evaluated: mean velocity (MV), ground reaction force (GRF) and sciatic func- tional index (SFI) using integrated equipment capable of digitally collecting the proposed data. The mean results of the MV data obtained by groups C, I and PBM were compared and a statistically significant difference was found, being: C x I (p=0.001) and I x PBM (p=0.002), respectively. Considering the SFI data obtained by groups C, I and PBM, a statisti- cally significant difference was observed when comparing C x I (p = 0.011). The mean results of the GRF data obtained by groups C, I and PBM were compared and the results showed a statistically significant difference in C x I (p=0.001), C x PBM (p=0.026) and I x PBM (p=0.002), respectively. Therefore, there was an improvement in motor function between the 7th and 14th day after SCI, represented by the increase in speed and better GRF distribution in the PBM group.Item Histological analysis of Spinal Cord Injury treated with Amniotic Membrane(Universidade de São Paulo) Correia, Débora Campos Chaves; Lima, Leonardo Borges de; Sant'Anna, Luciana Barros; Lima, Mario Oliveira; Arisawa, Emilia Angela Lo SchiavoSpinal cord injury (SCI) is one of the most harmful syndromes that affects humans due to neuronal destruction and interruption of the nerve impulse transmission between axons. The conduction of motor, sensory, and autonomic responses below the level of the injury is seriously compromised, generating high treatment costs for the health system and a reduction in quality of life, stimulating research into new treatment protocols. This study aimed to evaluate the effectiveness of a biomaterial, the amniotic membrane (AM), to treat experimentally induced SCI. 15 adult rats were divided into three groups (n = 5): S (Sham), L (SCI without treatment), and AM (SCI treated with AM). Spinal cord injury was induced in the region T9-T10 by direct trauma, free-falling a weight (10 g, 2 mm flat edge) held on a mini guillotine, 25 mm above the exposed spinal cord. A fragment of AM, obtained from the human placenta after maternal consent, was applied to the injured area only in the AM group. After 28 days, specimens from the area of spinal cord injury were excised and subjected to routine histological procedures. Data from the semi-quantitative score, obtained from a scheme that assigned different scores to regions of the spinal cord, and from the quantitative analysis were subjected to parametric statistical analysis. Results showed that Group S presented medullary tissue without changes (score 0). In contrast, Group L presented numerous areas of cavitation in the dorsal and lateral regions of the white and gray matter (9.61 ± 6.60 p<0.001) with an intense inflammatory infiltrate. The AM group exhibited small areas of cavitation in the dorsal and lateral regions of the white matter and part of the dorsal columns in the gray matter (0.94 ± 1,03, p<0.001), with few inflammatory cells. The results suggest the effectiveness of AM in the treatment of induced SCI, characterized by a reduction in the evolution of inflammatory and degenerative processes in the central nervous tissue compared to the untreated group.Item Therapeutic Potential of Photobiomodulation in Early Recovery After Experimental Spinal Cord Injury in Rats: Histological and Biomechanical Analysis(Wiley) Correia, Débora Campos Chaves; Lima, Leonardo Borges de; Lima, Mário Oliveira; Beloni, Luís Filipe Karatanasov; Hage, Raduan; Arisawa, Emilia Angela Lo SchiavoSpinal cord injury (SCI) leads to severe functional deficits, underscoring the critical need for new therapies. This study evaluated the efficacy of photobiomodulation (PBM) as an early, noninvasive treatment for induced SCI, using specific parameters (808 nm, 72J/cm2, 100mW). A total of 15 rats were divided into Control (C), SCI, and PBM groups. Efficacy was determined by an inte- grated approach, correlating ground reaction force (GRF) with quantitative histological assessment. The PBM group showed a significant reduction of secondary damage (33.3 ± 5.5 vs. 53.9 ± 7.0 in the SCI group; p = 0.0002) and preserved neural structure. This tissue preservation aligns with the GRF analysis, which demonstrated that the PBM group recovered gait patterns similar to the Control group. In conclusion, PBM effectively mitigates necrosis aerea, maintaining tissue integrity, improving functional recovery, and reinforcing the PBM's therapeutic potential as a promising translatable strategy for outcomes after SCI. Further research should include immunostaining of cells and larger samples.Item Photobiomodulation and amniotic membrane for treat tendon injury in rats(Academia Brasileira de Ciências) Nicodemo, Mariana de Castro; Arisawa, Emilia Angela Lo Schiavo; Sant’Anna, Luciana Barros; Martins, Rodrigo Álvaro Brandão LopesTendons, complex fibrous structures, are subjected to great tensions, which can give rise to the so-called tendinopathies. This study aimed to evaluate photobiomodulation and human Amniotic Membrane applied as single or combined therapies to treat induced Achilles tendon lesions. Seventy-five rats were divided into five groups (n=15): C- control Sham surgery; I- tendon injury; LA- tendon injury treated with photobiomodulation; AM- tendon injury treated with Amniotic Membrane; LAM- tendon injury + photobiomodulation and Amniotic Membrane, subdivided into three groups (n=5) with analysis at 3, 7, and 14 days. The tendon injuries were made with a 20 g weight released from a mini guillotine onto the ankle in dorsiflexion. AM and LAM groups received an Amniotic Membrane fragment while LA and LAM groups received transcutaneous photobiomodulation, using a 660 nm wavelength laser. The inflammatory cells showed statistical differences between groups C and I (p<0.05), I and AM (p<0.01), I and LA (p<0.05), and I and LAM (p<0.01). Both photobiomodulation and Amniotic Membrane were shown to enhance tendon repair, and the association of photobiomodulation plus Amniotic Membrane was the most effective treatment. We conclude that the association of photobiomodulation plus Amniotic Membrane was effective in accelerating and improving the tendon regeneration process.